Technical summary Essay Example | Topics and Well Written Essays - 500 words

Technical summary - Essay Example Mouse-adapted H1N1 A/PR/8/34 strains were used for the study. Genomic RNA (full-length) was acquired by infecting MDCK cells; the procedure was done under biosafety level-2 conditions. pFBHAhisKDEL and pFBMelHAhis plasmids were used to produce the recombinant baculoviruses BacHAhisKDEL and BacMelHAhis using Bac-to-Bac baculovirus expression system. The instructions provided by the manufacturer of the system were strictly followed. Control used was baculovirus vector BacNI (this is a baculovirus, which has no foreign gene). There was propagation and amplification of the recombinant baculoviruses in Sf21 insect cells in order to attain infective titers of around 108 plaque-forming units (pfu) (Gomez-Casado 36). Sf21 insect cells and the insect larvae (Trichoplusia ni) were infected using recombinant baculovirus dilution to attain the amount of pfu per dose required for each selection. Total soluble and non-denatured proteins (TSNDPs) were obtained through homogenization; these proteins were from baculoviruses infected T. ni larvae (Gomez-Casado 36). The proteins were prepared using various techniques for western blot (WB) assays and protein size determination. Recombinant HAhisKDEL protein purification from the infected larvae was done using Co2+-based immobilized metal affinity chromatography (IMAC) resins (Gomez-Casado 37). The response of HA-specific IgG (immunoglobin G) was measured using ELISA tests. Inhibition tests of hemagglutination were done for each of the duplicate based on instruction from the World Organization for Animal Health. Female mice (6 to 8 weeks old) were immunized and tested for virus challenge. The first group of mice (4) was immunized with TSNDP extract (containi ng HAhisKDEL protein) from the infected larvae. The second group (4 mice) was immunized with purified HAhisKDEL protein. Control group (3 mice) were immunized using TSNDP extract

Should Animal Experimentation Be Permitted Essay

Should Animal Experimentation Be Permitted - Essay Example Experiments on the regeneration of spinal nerve endings were conducted at the University of Zurich. In test-tube testing, nerves dismembered from rats were left open to immunoglobulin that blocked regeneration. The nerves regenerated successfully though not fully. The rats were the animals of choice because there was no other suitable replacement. The use of animals in these studies is important as those involved cannot present a raw form of treatment directly to humans. Opposition of experiments conducted on animals is a threat to medical advances and the furtherance of human knowledge. In relation to medical research, since there are no suitable replacements for animals, animal experimentation should be allowed. On the hand, due to the emotions attached individuals to animals, activists call for a ban as this is a selfish act for humans. The claim also is that, â€Å"will humans keep experimenting and murdering animals for these selfish gains till they are endangered?† Moreo ver, still on medical advances, animal testing should not be permitted as the same animals do not have the complexity of the human body (Murnaghan, par 3). Thus, should the products tested on animals fail on humans; it will be a waste of animals such as rats and mice. As a replacement for animals, computer models and simulations can be used to predict the likely reactions from an experiment (Murnaghan, par 3). Animal experiments should not be permitted if at all they are carried out unnecessarily (Environment commission, p.1). This is, in cases, such as those carried out for aesthetic value where animals should not be allowed for use. Instead, they should test their products by looking for volunteers, as it is the humans to use the products. At the same time, it tests on animals should be allowed as the animals conditions are put into considerations during the tests. In this case, they are not to suffer according to the law. This is such that, when the animals acquire adverse condit ions and complications from the experiments or reach the â€Å"human endpoint† (serious respiratory problems, loss of weight and extreme tumor growth), the animal is euthanized if the case is severe (Vos, p. 1). This is to mean that as long the animals welfare is taken into account, testing should be allowed. In drug experiment, animal testing assists in ensuring the wellbeing of medication taken by human beings or stuffs that human being are uncovered to frequently. Animals are used to test the dangers of these substances on humans thus lowering the potential harm and saving lives. Animal experiments should not be allowed as there are alternatives. Despite not being the best alternatives to animals, they can be used to reduce the numbers of animals used as well as the level of their suffering during experimentation. Replacements could be; cell cultures, which reduce the, number of animals used, use of statistics, which uses comprehensive data to gauge the spread of disease, and finally the use of advanced scanning technology that allows for investigation of disease through human scans rather than performing animal testing(Paul, 7). The use of animals for experimenting increases the knowledge and understanding of the animal being used for the experiment. This means that the animal species being used is a beneficiary of the experiment in that researchers may come up with ways to help the animals survive. This is as in cases where